分析物意义: 影响消化速率和淀粉利用的成分比例
Megazyme检测试剂盒优点: 试剂盒新颖，试剂稳定
For the measurement of amylose/amylopectin ratio and content in cereal starches and flours. based on a Con A precipitation procedure. Content:100 assays per kit
Colourimetric method for the determination of Amylose and
Amylopectin in cereals, food and feed
Principle:
(Con A)
(1) Soluble starch (amylose + amylopectin) →
amylose + amylopectin-Con A
(soluble) (precipitate)
(α-amylase + amyloglucosidase)
(2) Amylose (in solution) + H2O → D-glucose
(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(4) H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + H2O
(α-amylase + amyloglucosidase)
(5) Total starch (in solution) + H2O → D-glucose
Kit size: 100 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 120 min
Detection limit: Amylose 5-95% of total starch content
Application examples:
Cereal starches, flours, pure starches and foods
Method recognition: Novel method
Advantages
Very cost effective (cost per test)All reagents stable for > 12 months after preparation
only enzymatic kit available
Accurate and reliable amylose / amylopectin ratio determination
Simple format
Standard included